Eukariotic cell lines infection

The prerequisite for any bacterial infection is the colonization of host tissues. Pathogenic bacteria have thus developed an arsenal of molecules and structures capable of promote their attachment to host cells. This phenomenon, called bacterial adhesion, is an essential step before cell invasion or toxin secretion.

For your needs, SMALTIS has developed different models of cellular infections and different methods to quantify bacterial adhesion and / or invasion.

Our Models:

      - Intestinal cells infection models:
          - Available Cell lines: Caco2, T84, HT29
          - Relevant model to sudy Enterobacteria (E. coli, K. pneumoniae, ...)

      - Lung cells infection models:
          - Available Cell lines: A549 and NCI-H820
          - Relevant model to sudy P. aeruginosa, A. baumannii or S. pneumoniae

      - Macrophages infection model:
          - Available Cell lines: Murin Macrophages J774A.1
          - Relevant model to sudy phagocytosed bacteria and bacterial citotoxicity


Other models or cell lines on request.

Our method to quantify infection:

      - Counting method
      - Staining method with antibodies specific to the studied species

Examples of achivements:

      - Characteriaation by immuno-labeling of the adhesion and invasion properties of 100 strains of Escherichia coli on the T84 intestinal line
      - Assessment of adhesion/invasion properties of environmental strains (Pantoea sp, Arthobacter sp, Bacillus subtilis)
      - Charaterization of mutants derived from Pseudomonas aeruginosa PAO1 strain on A549 and NCI-H820 lung cell lines
      - Intracellular activity of antibacterial compounds on S. aureus strains using macrophages J774A.1
      - Determination of Lethal Concentration 50 (LC50) of different antibacterial molecules

                                                                                        Request a quote
Our services:

Cytotoxicity assessment
Cellular Response