Example of achievement

"Green Bacteria": construction of a platform strain from Pseudomonas aeruginosa (SM59 model)

WORK
From the reference P. aeruginosa strain PAO1, removal of most genetic elements confering antibiotic resistance, virulence and pathogenicity (efflux system, enzymes, membrane modification genes, secreted and structural factors).
approx. 1% of the genome removed
More informations about background of the project: R&D-Disarming Pseudomonas

CHARACTERISTICS
>   Unaltered fitness

>   Hypersensitivity to antibiotics: 
• 2 to 256-fold reduction in resistance to anti-pyocyanic antibiotics

>   Decrease in virulence & pathogenicity: 
• 68% ↓ in cytotoxicity to murine macrophages
• 44% ↓ in lung cell adhesion
• 74% ↓ in ability to form biofilm
• 50% ↓ in ability to kill Galleria mellonella larvae

Deleted mechanisms of resistance classified by family:
      - Efflux systems: MexAB, MexXY, MexCD, MexEF, MuxABC
      - Enzymes: AmpC, APH3’llb
      - Membrane modification system: ArnBCDATEF

Deleted mechanisms of virulence:
      - Elastase: LasA, LasB, AprA
      - Exotoxin: ToxA, ExoS, ExoT and ExoY
      - Flagellum: FliEFG
      - Phospholipase C: PlcH
      - Type IV Pilus: PilQ
      - Rhamnolipid: RhlA
      - Quorum Sensing: PqsA, RhlIR, LasIR
      - Lectin: LecA, LecB

List of available mutants:
Simple mutants:
      - PAO1∆mexXY
      - PAO1∆mexAB
      - PAO1∆mexCD 
      - PAO1∆mexEF
      - PAO1∆ampC
      - PAO1∆aph
      - PAO1∆muxABC
      - PAO1∆arnBCDATEF-ugd
      - PAO1∆fliEFG
      - PAO1∆pilQ
      - PAO1∆exoS
      - PAO1∆exoT
      - PAO1∆exoY
      - PAO1∆toxA
      - PAO1∆plcH
      - PAO1∆rhlA
      - PAO1∆pqsA
      - PAO1∆pcrVHpopBD
      - PAO1∆rhlIR
      - PAO1∆lasIR
      - PAO1∆lecA
      - PAO1∆lecB

Complex mutants (several inactivated genes) are also available. Feel free to contact us to get the complete list.