Beyond the gene deletion or replacement, we offer you to insert DNA fragments directly into the chromosome of your strains, thus creating new metabolic pathways.
These chromosome insertions are utterly stable and do not require any selection pressure (antibiotic or other) to be maintained in the long time. The creation of such strains may be considered a real alternative to the use of expression plasmids to produce recombinant proteins.
Thanks to our tools, we can also “tag” your strains by inserting a short DNA sequence in a specific spot of the chromosome. We should then be able to differentiate them among other strains of the same species.
Example of achivements:
- Creation of a bank of glow and fluorescent strains from so-called ESKAPE species (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter Baumannii, Pseudomonas aeruginosa and Enterobacter).
Mutagenesis directed straight to the chromosome