Genotyping

Molecular genotyping is the method used for epidemiological monitoring of pathogenic microorganisms, the production quality controls or identification of contamination sources. Thanks to various methods, SMALTIS enables you to determine the genotype of your strains and thus to verify their clonality (to verify whether or not you are dealing with the same strain)

Our methods:
 
      Genotyping of bacterial strain by Multiple-Locus Variable number tandem repeat Analysis (MLVA)

Genotyping by MLVA (Multi-Locus Variable number tandem repeat Analysis) is based upon the amplification by PCR (polymerase chain reaction) of various VNTRs (Variable Number of Tandem Repeats) scattered on the bacterial genome using specific primers. The determination of the molecular size of different amplicons by electrophoresis allows to identify the number of repetitions at a specific locus. By this manner, these results reflect the number of repeated units in the amplified region as the length of repeated units is known. In some species, this method significantly increases the level of bacterial genotyping. Typing results in a numeric code, which includes the number of patterns at each locus.


 
     Genotyping of bacterial strain by Pulsed Field Gel Electrophoresis (PFGE)

Genotyping by PFGE (Pulsed Field Gel Electrophoresis) allows to analyze the macrorestriction profiles of total DNA by pulsed field gel electrophoresis. The result is a migration profile, which defines a pulsotype characteristic of a bacterial isolate. PFGE is a standard method of typing for numerous bacterial species due to its highly discriminating potential.

PFGE                                                                                                                                                                                                                             Request a quote

Our services:

Identification of microorganism
Phenotyping
Evaluation of antibiotic resistance
Measurement of virulence factors