Identification of microorganisms

As a first step of a microbiological study, SMALTIS is able to accurately identify a wide variety of microorgansims, whether you are dealing with bacteria, yeasts or molds. At SMALTIS we offer you a wide range of identification solutions thanks to molecular biology tools. Our methods depend on the type(s) of microorganisms you wish to identify.

Our Methods:

      BRUKER MALDI-TOF Mass Spectrometry identification

The mass spectometry is an analytical method allowing the identification of the gender and the species of microorganisms in just a few minutes. This fast method, dependent on databases, is well adapted to the identification of most bacteria.

At SMALTIS we are also capable of setting up databases specific to your targeted strains

      Identification by Sanger Sequencing

Sanger-Sequencing is useful for the accurate identification of unknown and/or non-cultivable strains for which mass spectrometry is not adapted (e.g. Yeast and Mold strains). Depending on the microorganism, we amplify and sequence genes specific targets. The identification is then performed by comparing the targeted sequences with international databases

      Identification by Next-Generation Sequencing (NGS)

It can be use either from an isolated microorganism or from a microbiome, i.e a well characterised environment

To identify an isolated microorganism, whole genome sequencing is perform to obtain the raw data of the entire genome of the strain allowing both to identify the species and to obtain its genetic map

For a microbiota (i.e all the microorganisms present in a microbiome) the useful method consists in sequencing 16S rRNA gene, a highly conserved component of the transcriptional machinery of all DNA-based life forms

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Our services:

Phenotyping
Genotyping
Evaluation of Antibiotic Resistance
Measurement of Virulence Factors