Mutagenesis directly on the chromosome
In order to check the impact of a mutation on the expression of your genes or the functionality of your proteins of interest, we are able to modify your chosen nucleotides directly on the chromosome of your strain. The insertion of stable mutation will allow you not only to study the functionality of the variants of the same protein, but also to optimize the functionality of an enzyme by changing its active site.
Example of achivement:
- Modification of specific nucleotides in the coding gene for the AmpC beta-lactamase of Pseudomonas aeruginosa to assess the impact of potential mutations on the activity of the enzyme.
In order to know what we are able to do in relation to your strains, feel free to contact us.