Preparation of nucleic acids

Extraction and prepration of genomic DNA

The preparation of pure genomic DNA is a prerequisite for many applications such as amplification, sequencing or preparation of DNA standards for qPCR experiments

Depending on your needs, our molecular biology platform can support the extraction and purification of gDNA from your samples, whether they are blood, cell pellets, microorganisms, biopsies or even complex samples (sludge, soil, faeces, PAXgene® tubes, ...). For this, we adapt the extraction procedure to your samples.

The extracted DNA molecules are then:
      - Quantified by absorbance measurement using a NanoQuant Plate TM (TECAN)
      - Controlled by visualization of the electrophoretic profile on agarose gel
      - Preserved under controlled temperature

Our production capacities range from 1 to 200 samples simultaneously.

Extraction and prepration of RNA

The preparation of High-quality RNA is a critical step for transcrptiomic analyses such as the assessment of gene expression by RT-qPCR, RNA-sequencing, Microaray or metatranscriptomic analyses. 

In order to guarentee optimum results, our technical team can assume the extraction and purification of total RNA from your samples, whether they are blood, cell pellets, microorganisms, biopsies or even complex samples (sludge, soil, faeces, PAXgene® tubes, ...). 

The extracted RNA molecules are then: 
      - Quantified by absorbance measurement using a NanoQuant Plate TM (TECAN)
      - Controlled by capillary electroproresis (Bioanalyzer Platform 2100, AGILENT® or Experion, BIORAD®). The quality of RNAs is thus associated with a value between 0 and 10 (RIN or equivalent)
      - Preserved under controlled temperature

For your Gene Profiling (RNA-sequencing or Metatranscriptomic) experiments, we can also perform the ribo-depletion step, a prerequisite for the elimination of ribosomal RNAs.

Our production capacities range from 1 to 200 samples simultaneously.

Production of Plasmid DNA

The production of plasmid DNA in sufficient quantity and quality for applications such as cell transfection, production of qPCR DNA ranges or pre-clinical studies may be difficult to achieve.

Thus, we can assume the production of your plasmids by intervenung at differents levels: 
      - Vector Construction
      - Bacterial Transformation
      - Plamids production from a few micrograms to several milligrams (endo-free quality; <0.1 EU / μG of plasmid DNA)
      - Quality controls

Once produced, plasmids are then:
      - Quantified by absorbance measurement using a NanoQuant Plate TM (TECAN)
      - Adjuted to the desire concentration
      - Controlled by verefication of restriction profiles and/or sequencing
      - Preserved under controlled temperature before sending

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Our services:

Amplification of Nucleic acids
Vector Construction