Techniques

                                

                                

INTERNAL CAPACITIES

MICROBIOLOGY

›        Microorganisms characterization:
           • identification
           • phenotyping: morphology - physiological & metabolic traits 
           • molecular genotyping
           • antibiotic resistance evaluation
           • production of virulence factors measurement

›       Quantification & Isolation of bacteria by culture on specific media

›       Modification of bacterial genomes

MOLECULAR BIOLOGY

›        Preparation of nucleic acids

›        Amplification and sequencing of specific sequences
           by PCR, amplification of circular DNA by Rolling Circle Amplification (RCA PCR)


›        Vector construction

CELLULAR BIOLOGY

›        Adhesion and Invasion tests:
           • various cellular models:  intestinal, pulmonary and macrophages cells
           • cellular response evaluation
           • quantification by bacterial enumeration or immunolabelling

›        Evaluation of chemical or bacterial cytotoxicity

›        Cellular Infection and Response