Techniques






INTERNAL CAPACITIES
MICROBIOLOGY

› Microorganisms characterization:
• identification
• phenotyping: morphology - physiological & metabolic traits
• molecular genotyping
• antibiotic resistance evaluation
• production of virulence factors measurement
› Quantification & Isolation of bacteria by culture on specific media
› Modification of bacterial genomes

MOLECULAR BIOLOGY
› Preparation of nucleic acids
› Amplification and sequencing of specific sequences
by PCR, amplification of circular DNA by Rolling Circle Amplification (RCA PCR)
› Vector construction
CELLULAR BIOLOGY
› Adhesion and Invasion tests:

• various cellular models: intestinal, pulmonary and macrophages cells
• cellular response evaluation
• quantification by bacterial enumeration or immunolabelling
› Evaluation of chemical or bacterial cytotoxicity
› Cellular Infection and Response