Vectors construction

Obtaining a recombinant plasmid to produce plasmid DNA in large quantities, a recombinant protein or a recombinant vector can be long and fastidious.
SMALTIS can assume the construction of your vectors, whatever the final application. For that we offer you a personalized service.

Custom cloning service realised step by step including:

      - Selection and design of the targetted sequence
      - Optimization of the sequence according to your specifications
      - Selection of DNA synthesis (PCR or gene systhesis)
      - Diverse cloning strategies (Restriction, Gateway, SLIC)
      - Large choice of plasmids
      - Verification of final plasmid by restriction profiles and sequencing

Deliverables:

      - The bacterial strain containing the plasmid. The sending will be done according to the legislation
      - Plamsid DNA prepared according standard methods (See section Prepration of Nucleic Acids)

Associated Services:

      - Site-Directed Mutagenesis

Examples of achivements:

      - Construction of bacterial gene-inactivating plasmids
      - Construction of an expression plasmid to produce Gag, Pol and Env proteins from HERV virus
      - Construction of a Broad-Host range to produce recombinante proteins in Gram negative bacteria

                                                               Request a quote
Our services: 

Prepration of Nucleic Acids
Amplification of Nucleic Acids