In vitro evaluation of toxicity

· Determination of Minimal Inhibitory Concentration (MIC) at different pH
-depending on the way of action of the compound
-allows to mimic intracellular conditions
· Determination of Lethal Concentration 50 (LC50)
-concentration able to kill 50% of eukaryotic cells
· Evaluation of hemolytic activity
-Red Blood Cell Lysis: spectrophotometric measurement of free-hemoglobin
-red blood cells of different origins (human, sheep, mice…)
-concentrations close to those used in therapy